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Journal: Communications Biology
Article Title: Multiplex, high-throughput method to study cancer and immune cell mechanotransduction
doi: 10.1038/s42003-024-06327-x
Figure Lengend Snippet: PC3 cells exposed to 5000 shearing cycles using the VIAFLO96 equipped with 22 G needles with TRAIL treatment. Summary data for the percentage of ( a ) viable and ( b ) apoptotic PC3 cells. c Summary data for the percentage of depolarized mitochondria. Samples are stained manually or using the semi-automated program produced using VIALINK. d Representative AV/PI flow cytometry plots. e TRAIL sensitization from the viability generated in ( a ) were calculated using Eqs. and . ( n = 4 independent experiments ( a , b , e ), n = 3 independent experiments ( c ); two-way ANOVA test ( a – c , e ), (Supplementary Data )). * p < 0.05, ** p < 0.01, *** p < 0.005, **** p < 0.0001. Error bars represent ± SEM.
Article Snippet: The
Techniques: Staining, Produced, Flow Cytometry, Generated